Chemically Activated Fiber Tip and Fiber Optics, Complete with XY Control and Dichroic Mirror
The Fiber Optics Sensor (FOS), or Chemically Activated Fiber Tip, allows for the measurement to be performed in-situ, which is particularly of interest when the sample to be monitored is remote or is embedded in hazardous conditions (high temperature, toxic, flammable, high voltage, etc.). For instance, the accessory can be used to detect and map the presence of specific molecules inside the body (endoscope); or at different depths of the ocean; or to detect the presence of molecules in bioreactors.
The device consists of a fiber that channels using proper optics, the light produced by the spectrofluorometer's laser and carries it to the other end of the fiber, where a specific fluorophore is embedded in a polymeric matrix. The light excites the fluorophore; a portion of the fluorescence is carried back, through the same fiber, to the instrument that monitors its properties (intensity, lifetime), which depend upon the environment. The fiber, of about 100 Ám in diameter, can be up to 250 meters long, thus allowing for the monitoring of relatively far events in real time.
|objective||. Model LUCPlan FLN 20x/0.45 NA (by Olympus)
Any RMS-thread objective
|nanopositioning stage||. 3 mm overall adjustment
|aligning fiber||. 2 meter graded index 62.5 Ám Multimode Patch Cable (FC connector)
The RV Knorr is the research vessel owned by the U.S. Navy and operated by the Woods Hole Oceanographic Institution. A ChronosFD with the Fiber Optics Sensor has been installed on her (see R.B. Thompson et al., Methods in Enzymology, vol. 450; Elsevier 2008; pag. 311-337).
Selected References for the Use of Chemically Activated Fiber Tip and Fiber Optics in Various Research Areas
| Instrumentation for Fluorescence-based Fiber Optic Biosensors
Thompson, R.B., Zeng, H.-H., Ohnemus, D., McCranor, B., Cramer, M., Moffett, J.
Methods in Enzymology, Elsevier 2008, 450, 311-337.
| Real-time determination of picomolar free Cu(II) in seawater using a fluorescence-based fiber optic biosensor
Zeng, H.-H., Thompson, R.B., Maliwal, B.P., Fones, G.R., Moffett, J.W., Fierke, C.A.
Anal Chem., 2003, 75, 6807-6812.