TCSPC

ISS utilizes the high performance SPC-series of cards by Becker and Hickl for Time-Correlated Single Photon Counting (TCSPC) data acquisition. Inserted in the PCI bus of the computer, the card features a high resolution time bin (813 fs) and on-board constant fraction discriminators for the photon signal and the synchronization signal. The cards operational parameters are fully controlled through the ISS VistaVision software which includes the data analysis using fitting routines and the phasor analysis.

Measurements

  • Time-resolved measurements (FLIM and PLIM)
  • Single-wavelength FLIM, multi-wavelength FLIM
  • Confocal images
  • Polarization images (steady-state and time-resolved)
  • FCS, FCCS, PCH
  • Scanning FCS, RICS, N&B
  • Single Molecule FRET
  • PIE measurements

Features

  • 4-channel simultaneous acquisition using a router or using additional modules
  • Direct input from PMTs
  • Time channel resolution: 813 fs
  • Electrical time resolution down to 7 ps FWHM; 4 ps RMS
  • Dead time: 100 ns
  • Trigger out to synchronize external devices
  • Trigger input from external source
  • Line, Frame and Pixel CLK synchronization
  • Drivers for Windows 10 OS
Specifications
Feature Description
Photon Channel
No. of channels 1 independent channel
  • Up to 4 channels can be acquired using a router
  • Multiple cards can be used in parallel
Time resolution (FWHM/RMS) 6.6 ps / 2.5 ps
Input voltage range -30 mV to - 1 V
Min input pulse width 400 ps
Threshold 0 to -500 mV
Zero Cross Adj. -100 mV to +100 mV
Synchronization Channel
Input voltage range -30 mV to -1 V
Min input pulse width 400 ps
Threshold 0 to -500 mV
Zero Cross Adj. -100 mV to +100 mV
Frequency Divider 1-2-4
Frequency Range 0-150 MHz
Frequency Range Output voltage range: ± 12 V
Time-to-Amplitude Converters (TAC)
Principle Ramp Generator / Biased Amplifier
TAC range 50 ns to 5 µs
Biased Amplifier Gain 1 to 15
Biased Amplifier Offset 0 to 100% of TAC range
Min time per channel 813 fs

Data Acquisition (Histogram Mode)
Dead time 100 ns
Useful count rate 5 MHz
Saturated counts rate 10 MHz
Collection time 0.1 µs to 100,000 s
Display Interval time 0.1 µs to 100,000 s
Repeat time 0.1 µs to 100,000 s
Max counts per Time Channel 216 - 1
Synchronization with scanning Pixel, Line and Frame CLKs

Data Acquisition (Time-Tagged)
Dead time 100 ns
Saturated counts rate 10 MHz
Macro time resolution, internal CLK 50 ns, 12 bit
Macro time resolution, external CLK 10 ns to 100 ns, 12 bit
Experiment trigger TTL

Data Acquisition (Time-Tagged Imaging)
Dead time 100 ns
Saturated counts rate 10 MHz
Image Resolution  
    No. of time channels 64 256 1024 4096
    No. of pixels 4096 x 4096 2048 x 2048 1024 x 1024 512 x 512
Synchronization with scanning Pixel, Line and Frame CLKs

Operation
Architecture PCI bus (Windows 7, Windows 10)
Power 12 w from +5 V; 0.7 W from +12 V
Dimensions (mm) 240 (L) x 130 (W) x 15 (D)