Images acquired through Vista - FCS and Confocal FLIM Microscopy can be processed to display the fluorescence intensity profile. The color-palette is user-selectable; the user can also introduce the threshold to visualize regions of weak fluorescence or to enhance the signal versus background. By moving the cursor across the image, the intensity at each pixel is displayed, and the pixel with the maximum intensity is identified within the image. Intensity profiles along the X- and Y-axis can be displayed in a separate window that allows fitting of the PSF parameters.
Fluorescence intensity profile measured along the horizontal axis.
Fluorescence intensity profile measured along the vertical axis.