Frequency-domain anisotropy decays (Differential Polarized Phase Angle and Amplitude Ratio) of Coumarin 6 in ethylene glycol measured on ChronosFD using a 473-nm laser diode. The emission was collected using a 500-nm long-pass filter. Calculated values for θ = 2.0 ns with R0 = 0.38 and τ = 2.33 ns, T = 27-28°C.