Microwell Plate Reader

Fluorescence > Accessories > Microwell Plate Reader

Overview
Specifications
Measurement Examples

Microwell Plate Reader ••••
The microwell plate reader allows for the detection of fluorescent signal of the samples in microwell plates (microplates, microtiter plates). It is ideal for the experiments when such parameters, like number of samples measured per hour and cost per sample become important.

The ISS Microwell Plate Reader Model K428 can be easily attached to the fluorometers as a replacement for the regular cuvette sample holder. The accessory can be used for steady-state, frequency-domain lifetime acquisition and time-domain lifetime acquisition with PC1, K2, ChronosFD and ChronosBH fluorometers. It is compatible with the common microplate format used in many research and diagnostic laboratories which is 96-well (matrix of 8 by 12 wells), that has a typical sample volume in the range from 100 to 200 microliters per well. It is also compatible with higher density microplates such as 384-well plates.

The light is brought to the microwell plate by using a fiber bundle; the common leg includes both excitation fibers and collection fibers. The collection fibers are connected to the emission channel of the spectrofluorometer. The excitation and collection of the fluorescence occur from the top of the well. The microwellplate is housed onto a computer-controlled XY holder; it positions each well under the common termination of the fiber. The acquisition time at each well is set by the user; also, the user can enter a random selection of the wells. The unit is controlled through the USB port of the computer.

Microwell Plate Reader

Microwell Plates Format	96, 384
Sample Excitation	From the top
Fluorescence Collection	From the top
Positioning Resolution of the Fiber Over the Wells	100 µm
Cell-to-cell Speed [from A(x) to A(x+1)]	< 2 seconds
Speed from A1 to A12 (stopping at each cell between)	≈ 34 seconds
AC Input	90 - 264 VAC, 47 - 63 Hz single phase
Max Power	2.4 W

Bifurcated Fiber Optics

Total Number of Fibers	To be determined
Fiber Core Diameter	150-200µm
Fiber Type	Silica Core/Silica Clad
Operating Wavelength Range	300-700nm
Fiber Numerical Aperture	0.22
Common Leg Termination	Ferrule (10mm x 25mm long)
Excitation Leg Termination	Ferrule (10mm x 25mm long)
Collecting Leg Termination	Ferrule (10mm x 25mm long)
Common Leg Configuration	round; the fibers coming from the excitation leg are randomly mixed with the collecting fibers; total diameter of the common leg is about 4.5mm.
Excitation Leg Termination	round; total diameter of the fibers is about 2mm
Collecting Leg Termination	round
Total Length of Assembly	1.8m
Length of Common End (without the bifurcation)	0.22
Sheathing	Standard Cabling

The plot shows the time-domain intensity decay curve of BodipyFL (500nM concentration water solution) acquired on ChronosBH with a Microwell Plate Reader using a pulsed laser diode excitation at 470 nm. The emission was collected through a OG530 long pass filter. The data is best fitted by a single-exponential decay time of 5.70 ns (χ² = 1.03).)

The plot shows the phase and modulation frequency response curves of BodipyFL (500nM concentration water solution) acquired on ChronosFD with a Microwell Plate Reader using a laser diode excitation at 470 nm. The emission was collected through a OG530 long pass filter. The data is best fitted by a single-exponential decay time of 5.75 ns (χ² = 1.10).