SpectralFLIM
Parallel 16-PMT Spectral FastFLIM
Parallel 16-PMT Spectral FastFLIM
The Nikon A1 and C2 confocal microscopes systems can be easily expanded to Fluorescence Lifetime Imaging (FLIM) and Fluorescence Correlation Spectroscopy (FCS) capability using the ISS Upgrade Kits. External detectors are added to the system; photon counting acquisition ensures sensitivity to low signals. Data is acquired using one of two modalities: FastFLIM (digital frequency domain); or SWISS card (digital TCSPC, time-domain). While the choice depends upon the user preference, FastFLIM provides directly data in a suitable format for phasor plot analysis. The package includes VistaVision for data acquisition and processing.
The Nikon FCS and FLIM Upgrade Kit includes the following items:
FastFLIM Unit or TCSPC Unit | Each unit accepts the output signals (via BNC) from the detectors and the synchronization signal from the Olympus confocal head. |
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Detectors |
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Laser Launcher (not required for multiphoton systems) | Available for 3-, 4- and 6-lasers. The lasers beams are superimposed and the output of the laser launcher is connected to the microscope by using a fiber optic. |
Computer Running VistaVision by ISS | High-performance computer, 32 GB RAM, with a 32" flat monitor |