Evident (Olympus)FV1000, FV1200, FV3000, & FVMPE-RS

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Overview of Evident (Olympus)

The FV1000, FV1200, FV3000 and FVMPE-RS confocal microscopes systems can be easily expanded to Fluorescence Lifetime Imaging (FLIM) and Fluorescence Correlation Spectroscopy (FCS) capability using the ISS Upgrade Kits. Photon counting detection ensures sensitivity to low signals. Internal detectors can be utilized or external detectors can be added to the system. Data is acquired using one of two modalities: FastFLIM (digital frequency domain); or SWISS card (digital TCSPC, time-domain). While the choice depends upon the user preference, FastFLIM provides directly data in a suitable format for phasor plot analysis. The package includes VistaVision for data acquisition and processing.

The Olympus FCS and FLIM Upgrade Kit includes the following items:

FastFLIM Unit or TCSPC Unit Each unit accepts the output signals (via BNC) from the detectors and the synchronization signal from the Olympus confocal head.
Detectors Options of:
  • Internal detectors
  • External 2-detector unit coupled to the fiber optic port of the microscope
  • External 2-detectors coupled to the confocal head of the microscope
External Detectors Fast PMTs, Hybrid PMTs, or SPADs.
Laser Launcher
(not required for multiphoton systems)		 Available for 3-, 4- and 6-lasers. The lasers beams are superimposed and the output of the laser launcher is connected to the microscope by using a fiber optic.
Computer Running VistaVision by ISS High-performance computer, 32 GB RAM, with a 32" flat monitor

Product Specifications for Evident (Olympus)

Acquisition and Analysis Software
  • VistaVision by ISS
Fluorescence Fluctuations Spectroscopy (FFS) Module Measurements
  • Fluorescence Correlation Spectroscopy (auto- and cross-correlation)
  • Photon Counting Histogram (PCH)
  • FFS measurements at target XYZ locations in an image
  • FLCS, Fluorescence Lifetime Correlation Spectroscopy
  • Scanning FCS
  • Number & Brightness (N&B)
Imaging Module Measurements
  • Single-point (intensity, polarization, lifetime)
  • Single plan and z-stack (polarization images, ratiometric, FLIM)
Superresolution Module
  • Particle tracking
  • Nanoimaging
FLIM images (digital frequency-domain) (single plane and z-stack)
  • Acquired in digital frequency-domain (DFD). The routine acquires simultaneously a FLIM image and a steady-state image.
FLIM images time-domain (single plane and z-stack)
  • Acquired in time-correlated single photon counting (TCSPC)
Single Molecule Module
  • Burst Analysis
  • FRET and Correlation Methods
  • PIE-FRET Methods
Light Sources
  • Laser diodes: 370 - 1000 nm
  • Ti:Sapphire lasers
Laser Launcher
  • Models for 3-, 4-, 6-lasers.
  • Light is delivered to the microscope through a single-mode fiber optic.
Input Channels
  • Two
Detectors
  • Internal
    • (original) PMTs
  • External
    • GaAs PMT (Model H7422P)
    • Hybrid PMT (Model R10467U)
CLK
  • Pixel, Line, Frame
FLIM Image Data Acquisition Minimum Dwell Time
  • 4 µs/pixel
Unit Control
  • USB
Computer
  • High-performance processor, 32 GB RAM, Windows 11, 64-bit
  • 32" monitor, 2556 x 1440 resolution
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Product Accessories for Evident (Olympus)

Product Resources

Configurations for Olympus LSM Upgrade

The upgrade can be carried out following one of two modalities:

  1. By adding two external PMTs (2-detector unit or Mini-TDU); this solution is preferred when the detectors are not available through Olympus (Figure 1).
  2. Utilize the internal PMTs of the system (Figure 2).
Schematic of Olympus LSM Upgrade with Frequency-Domain Capabilities Utilizing the FastFLIM module
Figure 1. Schematics of the upgrade package for the Olympus confocal microscope using modulated lasers and FastFLIM. The part to the right includes the Olympus instrument components (PC, control electronics, scanner and laser launcher). The left part of the schematics includes the components provided by ISS with the upgrade package. The detectors can be connected to the system confocal head or a fiber optics cable can bring the fluorescence signal to them.
Schematic of Olympus LSM Upgrade with Time-Domain Capabilities Utilizing the TCSPC module
Figure 2. Schematics of the upgrade package for the Olympus system using the internal detectors of the instrument. The part to the right includes the instrument components (PC, control electronics, scanner and laser launcher). The signal is collected from the internal detectors of the microscope and diverted to FastFLIM unit (left part) of the schematics which includes the components provided by ISS with the upgrade package.