单比色皿样本室
Overview of PC1
PC1是一个高度灵敏、小巧、计算机控制的光子计数光谱仪，专为物理化学、生物化学、生理学、神经化学、分子生物学、环境分析以及免疫分析研究中的应用打造设计。
PC1的关键特征
最大的灵敏度
全自动的
与外部设备集成
可升级的
数据减少管理
灵敏度 - 这款产品紧凑的设计优化了光路，使样品室可以尽可能收到最多的激发光，并且收集器能够高效收集荧光信号。这样的设计使得该仪器拥有超强的荧光团测定能力与极低的量子产量。
全自动化 - 仪器的控制与数据读取都是通过Vinci用PC控制完成的。所有参与的仪器组成部分和集成外部设备都会在仪器的控制窗口上显示，用户可以在此对所有设备和配件进行直接控制。
外部设备的整合 - PC1能够整合外部设备，例如停流配件、恒温浴、压力泵和滴定仪。其中任何一个设备都能被Vinci直接控制。
数据减少管理 - Vinci - 为了能与其他软件包最大兼容，多维荧光光谱软件获取到的数据为ASCII格式。数据文件可以被直接储存在其他与本地网络连接的工作站上。
可升级性 - PC1可以被完全升级至时间解析光谱仪，用于获取皮秒解析度下的时间解析荧光和磷光测定。它拥有各种光源和其他配件，可以被广泛地应用。
Product Specifications for PC1
测量 (稳态荧光)
- 矫正的激发和发射光谱
- 激发发射矩阵
- 偏振 (各向异性) 测量
- 光子计数模式下的毫秒动力学
- 双波长比激发或发射测定
光源
- 300 W 高压氙弧灯，在275 nm处为45 mW/nm
- 灯的电源供给：电流可控并且带有时间计
可选光源
- 激光二极管
- 发光二极管 (LEDs)
- 超连续光谱激光器 (氩离子、氪离子、氦镉等)
单色仪
- 单凹面全息光栅，其波长范围为从200 nm至1200 nm (取决于光栅的选择)
波长精确度
- ±0.2 nm
波长的可再现性
- ±0.25 nm
转换率
- 160 nm/s
棱镜
- UV级熔石英棱镜
偏振器
- UV级格兰汤普逊，10 x 10 mm, L/A=2.0
- UV级格兰汤普逊，14 x 14 mm, L/A=2.0
- UV级格兰汤泰勒，10 x 10 mm, L/A=2.0 (用于高功率激光器)
光学设计和收集几何排列
- 平行光设计用于精确偏振测量
- T式样以同时在2个发射通道上采集
探测器
- 波长范围：240 - 900 nm，上至1,700 nm (可选择)
探测模式
- 光子计数电子元件，10 KHz，在3个独立的通道上
- 可选：模拟输出
前置放大器鉴频器
- 80 MHz带宽，TTL输出
动态范围
- 线性上至4 million 次/秒
灵敏度
- 800 fM的荧光素 (带有冷却PMT外壳)
信号-噪点比
- 2000:1 (室温PMT外壳)
- 6000:1 (冷却PMT外壳)
自动化
- 4个快门的控制
- 上至3个单色仪
- 3个偏振器
- 样本支架旋转
- 搅拌器
- 滤光轮
至RS232设备的仪器接口
- 滴定仪
- 停流装置
- 帕尔贴样本室
- 流通式温度浴
操作系统
- Windows 11，64比特
电源要求
- 通用电源输入：110 - 240 V, 50/60 Hz, 400 VAC
大小 (mm)
- 885 (长) x 600 (宽) x 330 (高)
- 包括灯：885 (长) x 835 (宽) x 330 (高)
重量 (kg)
- 40
PC1的配置示例
PC1的产品配件
产品资源
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“Conserved Cysteine 126 in Triosephosphate Isomerase Is Required Not for Enzymatic Activity but for Proper Folding and Stability.” González-Mondragón, E., Zubillaga, R.A., Saavedra, E., Chánez-Cárdenas, M.E., Pérez-Montfort, R. & Hernández-Arana, A. Biochemistry, 43(11), pp. 3255–3263, 2004, Feb. doi: 10.1021/bi036077s.
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“Supersaturated Antisolvent-Assisted Crystallization for Highly Efficient Inorganic Perovskite Light-Emitting Diodes.” Kim, B.W., Im, S.H. ACS Nano, Volume 18, Issue 42(1), 2024, Oct. doi: 10.1021/acsnano.4c06465.
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“Polymer/Gold Nanoparticle Nanocomposite Light-Emitting Diodes: Enhancement of Electroluminescence Stability and Quantum Efficiency of Blue-Light-Emitting Polymers.” Park, J.H., Lim, Y.T., Park, O.O., Kim, J.K., Yu, J.-W. & Kim, Y.C. Chemistry of Materials, 16(4), pp. 688–692, 2004, Jan. doi: 10.1021/cm0304142.
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“Effects of Surface Passivation on Silicon Nanoparticle Photoluminescence.” Harwell, D.E., Croney, J.C., Qin, W., Thornton, J.T., Day, J.H., Hajime, E.K. & Jameson, D.M. Chemistry Letters, 32(12), pp. 1194–1195, 2003, Dec. doi: 10.1246/cl.2003.1194.
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“Photophysical characterization of [Ir(ppy)2(dmb)][PF6] towards application in light-emitting electrochemical cells (LECs).” Zanoni, K.P.S., Sanematsu, M.S. & Iha, N.Y.M. Inorganic Chemistry Communications, 43(8), pp. 162–164, 2014, May. doi: 10.1016/j.inoche.2014.02.010.
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“Blue-Green Iridium(III) Emitter and Comprehensive Photophysical Elucidation of Heteroleptic Cyclometalated Iridium(III) Complexes.” Zanoni, K.P.S., Kariyazaki, B.K., Ito, A., Brennaman, M.K., Meyer, T.J. & Iha, N.Y.M. Inorganic Chemistry, 53(8), pp. 4089–4099, 2014, Mar. doi: 10.1021/ic500070s.
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“White light emission from a polymer bilayer by incomplete cascade energy transfer.” Park, J.H., Park, O.O., Kim, J.K., Yu, J.-W. & Kim, Y.C. Current Applied Physics, 6(4), pp. 640–643, 2006, Jul. doi: 10.1016/j.cap.2005.04.011.
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“On the use of theoretical tools in the study of photophysical properties of the new Eu(fod)3 complex with diphenbipy.” Santos, E.R.d., Santos, M.A.d., Freire, R.O., Júnior, S.A., Barreto, L.S. & Mesquita, M.E.d. Chemical Physics Letters, 418(4-6), pp. 337–341, 2006, Feb. doi: 10.1016/j.cplett.2005.10.114.
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“Spectroscopic properties of the Eu(fod)3Phen–NO incorporated carboxylate glass.” Beltrão, M.A., Santos, M.L., Mesquita, M.E., Barreto, L.S., da Costa, N.B., Freire, R.O. & Couto dos Santos, M.A. Journal of Luminescence, 116(1-2), pp. 132–138, 2006, Jan. doi: 10.1016/j.jlumin.2005.04.002.
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“Remote and Adjacent Excited-State Electron Transfer at TiO2 Interfaces Sensitized to Visible Light with Ru(II) Compounds.” Liu, F. & Meyer, G.J. Inorganic Chemistry, 44(25), pp. 9305–9313, 2005, Nov. doi: 10.1021/ic0513336.
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“α-Crystallin binding in vitro to lipids from clear human lenses.” Grami, V., Marrero, Y., Huang, L., Tang, D., Yappert, M.C. & Borchman, D. Experimental Eye Research, 81(2), pp. 138–146, 2005, Aug. doi: 10.1016/j.exer.2004.12.014.
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“Spectroscopic Studies of the Intermolecular Interactions of Congo Red and Tinopal CBS with Modified Cellulose Fibers.” Yamaki, S.B., Barros, D.S., Garcia, C.M., Socoloski, P., Oliveira,, O.N. & Atvars, T.D.Z. Langmuir, 21(12), pp. 5414–5420, 2005, May. doi: 10.1021/la046842j.
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“Photophysical study of a conjugated–non-conjugated PPV-type electroluminescent copolymer.” Machado, A., Neto, J.D.M., Cossiello, R., Atvars, T., Ding, L., Karasz, F. & Akcelrud, L. Polymer, 46(8), pp. 2452–2460, 2005, Mar. doi: 10.1016/j.polymer.2005.02.007.
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“White polymer light-emitting devices from ternary-polymer blend with concentration gradient.” Park, J.H., Lee, T.-W., Kim, Y.C., Park, O.O. & Kim, J.K. Chemical Physics Letters, 403(4-6), pp. 293–297, 2005, Feb. doi: 10.1016/j.cplett.2004.12.119.
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“Hole-transporting property of a chemically hybridized poly(vinylcarbazole)-fullerene.” Park, J.H., Park, O.O., Kim, J., Yu, J.-W., Kim, J.K. & Kim, Y.C. Current Applied Physics, 4(6), pp. 659–662, 2004, Nov. doi: 10.1016/j.cap.2003.11.066.
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“Photophysical Study in Blends of Poly(alkyl methacrylate-co-styrene)/Polystyrene.” de Andrade, M.L. & Atvars, T.D.Z. Macromolecules, 37(24), pp. 9096–9108, 2004, Nov. doi: 10.1021/ma049265e.
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“Diffusion of a Single Component in a Binary Polymer Blend upon Annealing Monitored by Fluorescence Microspectroscopy.” de Andrade, M.L. & Atvars, T.D.Z. Macromolecules, 37(25), pp. 9626–9630, 2004, Nov. doi: 10.1021/ma048873r.
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“Optoelectronic and Photophysical Properties of Polyfluorene Blends as Side-Chain Length and Shape.” Byun, H.Y., Chung, I.J., Shim, H.-K. & Kim, C.Y. Macromolecules, 37(18), pp. 6945–6953, 2004, Aug. doi: 10.1021/ma049772w.
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“The effects of alkyl side-chain length and shape of polyfluorenes on the photoluminescence spectra and the fluorescence lifetimes of polyfluorene blends with poly(n-vinylcarbazole).” Byun, H.Y., Chung, I.J., Shim, H.-K. & Kim, C.Y. Chemical Physics Letters, 393(1-3), pp. 197–203, 2004, Jul. doi: 10.1016/j.cplett.2004.06.029.
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“Enhanced light output in bilayer light-emitting diodes with film thickness variations.” Park, J.H., Lee, K.-J., Park, O.O., Yu, J.-W., Kim, Y.C. & Kim, J.K. Chemical Physics Letters, 386(1-3), pp. 101–104, 2004, Mar. doi: 10.1016/j.cplett.2004.01.039.
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“Dynamic and Static Fluorescence Spectroscopy Applied to Miscibility of Poly(n-butyl methacrylate-co-styrene) with Polystyrene and Morphological Analysis by Epifluorescence Microscopy.” Andrade, M.L.d. & Atvars, T.D.Z. The Journal of Physical Chemistry B, 108(13), pp. 3975–3984, 2004, Mar. doi: 10.1021/jp034664h.
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“Surface Treatment to Enhance the Quantum Efficiency of Semiconductor Nanocrystals.” Jang, E., Jun, S., Chung, Y. & Pu, L. The Journal of Physical Chemistry B, 108(15), pp. 4597–4600, 2004, Mar. doi: 10.1021/jp049475t.
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“Photo and electroluminescence studies of poly(methyl methacrylate-co-9-anthryl methyl methacrylate).” Deus, J.F.d., Andrade, M.L., Atvars, T.D. & Akcelrud, L. Chemical Physics, 297(1-3), pp. 177–186, 2004, Feb. doi: 10.1016/j.chemphys.2003.10.023.
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“Orbital Control of the Color and Excited State Properties of Formylated and Fluorinated Derivatives of Azulene.” Shevyakov, S.V., Li, H., Muthyala, R., Asato, A.E., Croney, J.C., Jameson, D.M. & Liu, R.S.H. The Journal of Physical Chemistry A, 107(18), pp. 3295–3299, 2003, Apr. doi: 10.1021/jp021605f.
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“Immobilized Particle Arrays: Coalescence of Planar- and Suspension-Array Technologies.” Stevens, P.W., Wang, C.H.J. & Kelso, D.M. Analytical Chemistry, 75(5), pp. 1141–1146, 2003, Jan. doi: 10.1021/ac020580d.
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“Individual Metal Ligands Play Distinct Functional Roles in the Zinc Sensor Staphylococcus aureus CzrA.” Pennella, M.A., Arunkumar, A.I. & Giedroc, D.P. Journal of Molecular Biology, 356(5), pp. 1124–1136, 2006, Mar. doi: 10.1016/j.jmb.2005.12.019.
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“Structural Insights into Homo- and Heterotropic Allosteric Coupling in the Zinc Sensor S. aureus CzrA from Covalently Fused Dimers.” Lee, S., Arunkumar, A.I., Chen, X. & Giedroc, D.P. Journal of the American Chemical Society, 128(6), pp. 1937–1947, 2006, Jan. doi: 10.1021/ja0546828.
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“A high-throughput resonance energy transfer assay for Staphylococcus aureus DNA ligase.” Benson, E.L., Tomich, P.K., Wolfe, M.L., Choi, G.H., Hagadorn, J.C., Mutchler, V.T. & Garlick, R.L. Analytical Biochemistry, 324(2), pp. 298–300, 2004, Jan. doi: 10.1016/j.ab.2003.09.019.
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“Kinase Inhibitors: Not Just for Kinases Anymore.” Mcgovern, S.L. & Shoichet, B.K. Journal of Medicinal Chemistry, 46(8), pp. 1478–1483, 2003, Mar. doi: 10.1021/jm020427b.
