Data Tables
Lifetime Data of Selected Fluorophores
The following data tables provide you with information on the fluorescent lifetimes, excitation and emission wavelengths of Selected fluorescent dyes, probes and labels that are frequently used for biological applications and in biomedical research.
| Fluorophore | Lifetime [ns] | Solvent | Excitation max [nm] | Emission max [nm] |
|---|---|---|---|---|
| 5-Hydroxytryptamine | 370-415 | 520-540 | ||
| ATTO 565 | 3.4 | Water | 561 | 585 |
| ATTO 655 | 3.6 | Water | 655 | 690 |
| Acridine Orange | 2.0 | PB pH 7.8 | 500 | 530 |
| Acridine Yellow | 470 | 550 | ||
| Alexa Fluor 488 | 4.1 | PB pH 7.4 | 494 | 519 |
| Alexa Fluor 532 | 530 | 555 | ||
| Alexa Fluor 546 | 4.0 | PB pH 7.4 | 554 | 570 |
| Alexa Fluor 633 | 3.2 | Water | 621 | 639 |
| Alexa Fluor 647 | 1.0 | Water | 651 | 672 |
| Alexa Fluor 680 | 1.2 | PB pH 7.5 | 682 | 707 |
| BODIPY 500/510 | 508 | 515 | ||
| BODIPY 530/550 | 534 | 554 | ||
| BODIPY FL | 5.7 | Methanol | 502 | 510 |
| BODIPY TR-X | 5.4 | Methanol | 588 | 616 |
| Cascade Blue | 375 | 410 | ||
| Coumarin 6 | 2.5 | Ethanol | 460 | 505 |
| CY2 | 489 | 506 | ||
| CY3B | 2.8 | PBS | 558 | 572 |
| CY3 | 0.3 | PBS | 548 | 562 |
| CY3.5 | 0.5 | PBS | 581 | 596 |
| CY5 | 1.0 | PBS | 646 | 664 |
| CY5.5 | 1.0 | PBS | 675 | 694 |
| Dansyl | 340 | 520 | ||
| DAPI | 0.16 | TRIS/EDTA | 341 | 496 |
| DAPI + ssDNA | 1.88 | TRIS/EDTA | 358 | 456 |
| DAPI + dsDNA | 2.20 | TRIS/EDTA | 356 | 455 |
| DPH | 354 | 430 | ||
| Erythrosin | 529 | 554 | ||
| Ethidium Bromide - no DNA | 1.6 | TRIS/EDTA | 510 | 595 |
| Ethidium Bromide + ssDNA | 25.1 | TRIS/EDTA | 520 | 610 |
| Ethidium Bromide + dsDNA | 28.3 | TRIS/EDTA | 520 | 608 |
| FITC | 4.1 | PB pH 7.8 | 494 | 518 |
| Fluorescein | 4.0 | PB pH 7.5 | 495 | 517 |
| FURA-2 | 340-380 | 500-530 | ||
| GFP | 3.2 | Buffer pH 8 | 498 | 516 |
| Hoechst 33258 - no DNA | 0.2 | TRIS/EDTA | 337 | 508 |
| Hoechst 33258 + ssDNA | 1.22 | TRIS/EDTA | 349 | 466 |
| Hoechst 33258 + dsDNA | 1.94 | TRIS/EDTA | 349 | 458 |
| Hoechst 33342 - no DNA | 0.35 | TRIS/EDTA | 336 | 471 |
| Hoechst 33342 + ssDNA | 1.05 | TRIS/EDTA | 350 | 436 |
| Hoechst 33342 + dsDNA | 2.21 | TRIS/EDTA | 350 | 456 |
| HPTS | 5.4 | PB pH 7.8 | 454 | 511 |
| Indocyanine Green | 0.52 | Water | 780 | 820 |
| Laurdan | 364 | 497 | ||
| Lucifer Yellow | 5.7 | Water | 428 | 535 |
| Nile Red | 485 | 525 | ||
| Oregon Green 488 | 4.1 | Buffer pH 9 | 493 | 520 |
| Oregon Green 500 | 2.18 | Buffer pH 2 | 503 | 522 |
| Oregon Green 514 | 511 | 530 | ||
| Prodan | 1.41 | Water | 361 | 498 |
| Pyrene | > 100 | Water | 341 | 376 |
| Rhodamine 101 | 4.32 | Water | 496 | 520 |
| Rhodamine 110 | 4.0 | Water | 505 | 534 |
| Rhodamine 123 | 505 | 534 | ||
| Rhodamine 6G | 4.08 | Water | 525 | 555 |
| Rhodamine B | 1.68 | Water | 562 | 583 |
| Ru(bpy)3[PF6]2 | 600 | Water | 455 | 605 |
| Ru(bpy)2(dcpby)[PF6]2 | 375 | Buffer pH 7 | 458 | 650 |
| SeTau-380-NHS | 32.5 | Water | 270 | 480 |
| SeTau-404-NHS | 9.3 | Water | 402 | 515 |
| SeTau-405-NHS | 9.3 | Water | 405 | 518 |
| SeTau-425-NHS | 26.2 | Water | 340 425 |
545 |
| SITS | 336 | 438 | ||
| SNARF | 480 | 600-650 | ||
| Stilbene SITS, SITA | 365 | 460 | ||
| Texas Red | 4.2 | Water | 589 | 615 |
| TOTO-1 | 2.2 | Water | 514 | 533 |
| YOYO-1 no DNA | 2.1 | TRIS/EDTA | 457 | 549 |
| YOYO-1 + ssDNA | 1.67 | TRIS/EDTA | 490 | 510 |
| YOYO-1 + dsDNA | 2.3 | TRIS/EDTA | 490 | 507 |
| YOYO-3 | 612 | 631 |
PBS = phosphate buffered saline pH 7.4
PB = phosphate buffer
TRIS/EDTA (1mM, pH 7.4) = tris(hydroxymethyl)aminomethane/ethylenediamine-tetraacetic acid.
ss = single-stranded
ds = double-stranded
