PC1Photon-counting spectrometer

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Overview of PC1

PC1 is a highly sensitive, compact, computer-controlled photon-counting spectrometer designed for applications in physical chemistry, biochemistry, physiology, neurochemistry, molecular biology, environmental analysis, and immunoassay research.

Key Features of PC1

Maximum Sensitivity

Maximum Sensitivity

Fully Automated

Fully Automated

Integration of External Devices

Integration of External Devices

Upgradeable

Upgradeable

Data Reduction Management

Data Reduction Management

Sensitivity - The compact design optimizes the light path so that the sample compartment receives the maximum possible excitation light and the detectors collect the fluorescence signal with high efficiency. The end result of the compact design is an instrument with an outstanding ability to measure fluorophores with very low quantum yields.

Full Automation - Instrument control and data acquisition are PC-controlled through Vinci. All active instrument components and integrated external devices are displayed in the instrument control window where the user has direct control over all devices and accessories.

Integration of External Devices - PC1 has the capability to integrate external devices such as a stopped-flow accessory, temperature bath, pressure pump, and titrator. Any of these devices can be directly controlled through Vinci.

Data Reduction Management - Vinci - Multidimensional Fluorescence Spectroscopy software acquires data in ASCII format for maximum compatibility with other software packages. Data files can be stored directly on other workstations connected to a local area network.

Upgradability - PC1 is fully upgradable to a time-resolved spectrometer to acquire time-resolved fluorescence and phosphorescence measurements with picosecond resolution. A variety of light sources and other accessories are available for a broad range of applications.

Product Specifications for PC1

Measurements (Steady-State Fluorescence)
  • Corrected excitation and emission spectra
  • Excitation-emission matrices
  • Polarization (anisotropy) measurements
  • Millisecond kinetics in photon counting mode
  • Dual-wavelength-ratiometric excitation or emission measurements
Light Sources
  • 300 W high-pressure xenon arc lamp, 45 mW/nm brightness at 275 nm
  • Lamp power supply: controllable in current, with time meter
Optional Sources
  • Laser diodes
  • Light emitting diodes (LEDs)
  • Supercontinuum lasers (argon-ion, krypton-ion, helium-cadmium, etc.)
Monochromators
  • Single concave holographic gratings. Wavelength range: from 200 nm to 850 nm; optional: from 400 nm to 1,600 nm.
Wavelength Accuracy
  • ±0.2 nm
Wavelength Reproducibility
  • ±0.25 nm
Slew Rate
  • 160 nm/s
Lenses
  • UV-grade fused silica lenses
Polarizers
  • UV-grade Glan-Thompson, 10 x 10 mm, L/A=2.0
  • UV-grade Glan-Thompson, 14 x 14 mm, L/A=2.0
  • UV-grade Glan-Taylor, 10 x 10 mm, L/A=2.0 (for high power lasers)
Optical Design and Collection Geometry
  • Parallel beam design for precise polarization measurements
  • T-format for simultaneous acquisition on 2 emission channels
Detectors
  • Wavelength range: 240 - 900 nm, up to 1,700 nm (optional)
Detection Modes
  • Photon counting electronics, 10 KHz, on 3 independent channels
  • Optional: Analog Output
Pre-Amplifier Discriminators
  • 80 MHz bandwidth, TTL output
Dynamic Range
  • Linear up to 4 million counts/s
Sensitivity
  • 800 fM of fluorescein (with cooled PMT housing)
Signal-to-Noise Ratio
  • 2000:1 (room temperature PMT housing)
  • 6000:1 (cooled PMT housing)
Automation
  • Control of 4 shutters
  • Up to 3 monochromators
  • 3 polarizers
  • Sample holder rotation
  • Stirrers
  • Filter wheel
Instrument Interface to RS232 Devices
  • Titrators
  • Stopped-flow apparatus
  • Peltier sample compartment
  • Flow-through temperature bath
Operating System
  • Windows 10, 64-bit
Power Requirements
  • Universal power input: 110 - 240 V, 50/60 Hz, 400 VAC
Dimensions (mm)
  • 885 (L) x 600 (W) x 330 (H)
  • With lamp: 885 (L) x 835 (W) x 330 (H)
Weight
  • 40 kg
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Configuration for PC1

Configuration of PC1

Product Accessories for PC1

Product Software for PC1

Vinci

Vinci

A comprehensive multidimensional fluorescence spectroscopy software program designed to enhance the capabilities and performance of ISS spectrofluorometers.

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Product Resources