Overview of PC1
PC1 is a highly sensitive, compact, computer-controlled photon-counting spectrometer designed for applications in physical chemistry, biochemistry, physiology, neurochemistry, molecular biology, environmental analysis, and immunoassay research.
Key Features of PC1
Maximum Sensitivity
Fully Automated
Integration of External Devices
Upgradeable
Data Reduction Management
Sensitivity - The compact design optimizes the light path so that the sample compartment receives the maximum possible excitation light and the detectors collect the fluorescence signal with high efficiency. The end result of the compact design is an instrument with an outstanding ability to measure fluorophores with very low quantum yields.
Full Automation - Instrument control and data acquisition are PC-controlled through Vinci. All active instrument components and integrated external devices are displayed in the instrument control window where the user has direct control over all devices and accessories.
Integration of External Devices - PC1 has the capability to integrate external devices such as a stopped-flow accessory, temperature bath, pressure pump, and titrator. Any of these devices can be directly controlled through Vinci.
Data Reduction Management - Vinci - Multidimensional Fluorescence Spectroscopy software acquires data in ASCII format for maximum compatibility with other software packages. Data files can be stored directly on other workstations connected to a local area network.
Upgradability - PC1 is fully upgradable to a time-resolved spectrometer to acquire time-resolved fluorescence and phosphorescence measurements with picosecond resolution. A variety of light sources and other accessories are available for a broad range of applications.
Product Specifications for PC1
Measurements (Steady-State Fluorescence)
- Corrected excitation and emission spectra
- Excitation-emission matrices
- Polarization (anisotropy) measurements
- Millisecond kinetics in photon counting mode
- Dual-wavelength-ratiometric excitation or emission measurements
Light Sources
- 300 W high-pressure xenon arc lamp, 45 mW/nm brightness at 275 nm
- Lamp power supply: controllable in current, with time meter
Optional Sources
- Laser diodes
- Light emitting diodes (LEDs)
- Supercontinuum lasers (argon-ion, krypton-ion, helium-cadmium, etc.)
Monochromators
- Single concave holographic gratings. Wavelength range: from 200 nm to 850 nm; optional: from 400 nm to 1,600 nm.
Wavelength Accuracy
- ±0.2 nm
Wavelength Reproducibility
- ±0.25 nm
Slew Rate
- 160 nm/s
Lenses
- UV-grade fused silica lenses
Polarizers
- UV-grade Glan-Thompson, 10 x 10 mm, L/A=2.0
- UV-grade Glan-Thompson, 14 x 14 mm, L/A=2.0
- UV-grade Glan-Taylor, 10 x 10 mm, L/A=2.0 (for high power lasers)
Optical Design and Collection Geometry
- Parallel beam design for precise polarization measurements
- T-format for simultaneous acquisition on 2 emission channels
Detectors
- Wavelength range: 240 - 900 nm, up to 1,700 nm (optional)
Detection Modes
- Photon counting electronics, 10 KHz, on 3 independent channels
- Optional: Analog Output
Pre-Amplifier Discriminators
- 80 MHz bandwidth, TTL output
Dynamic Range
- Linear up to 4 million counts/s
Sensitivity
- 800 fM of fluorescein (with cooled PMT housing)
Signal-to-Noise Ratio
- 2000:1 (room temperature PMT housing)
- 6000:1 (cooled PMT housing)
Automation
- Control of 4 shutters
- Up to 3 monochromators
- 3 polarizers
- Sample holder rotation
- Stirrers
- Filter wheel
Instrument Interface to RS232 Devices
- Titrators
- Stopped-flow apparatus
- Peltier sample compartment
- Flow-through temperature bath
Operating System
- Windows 11, 64-bit
Power Requirements
- Universal power input: 110 - 240 V, 50/60 Hz, 400 VAC
Dimensions (mm)
- 885 (L) x 600 (W) x 330 (H)
- With lamp: 885 (L) x 835 (W) x 330 (H)
Weight (kg)
- 40
Example Configuration for PC1
Product Accessories for PC1
Product Software for PC1
Vinci
A comprehensive multidimensional fluorescence spectroscopy software program designed to enhance the capabilities and performance of ISS spectrofluorometers.
Learn MoreProduct Resources
- Absorption Measurements on PC1
- Anisotropy Decay Measurements
- Fluorescence Basic Instrumentation
- Fluorescence Lifetime
- Fluorescence Polarization
- Fluorescence Spectroscopy
- Long-Wavelength Polarization Standards
- Measurement of Fluorescence Quantum Yields on ISS Instrumentation Using Vinci
- Polarization Measurements: Parallel vs. Non-Parallel Beam Geometry
- Phasor Plots for the Analysis of Time-resolved Fluorescence
- What is Total Internal Reflection Fluorescence (TIRF)?
- A Critical Comparison of Xenon Lamps
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“Inhibition of yeast glutathione reductase by trehalose: possible implications in yeast survival and recovery from stress.” Sebollela, A., Louzada, P.R., Sola-Penna, M., Sarone-Williams, V., Coelho-Sampaio, T. & Ferreira, S.T. The International Journal of Biochemistry & Cell Biology, 36(5), pp. 900–908, 2004, May. doi: 10.1016/j.biocel.2003.10.006.
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“The Biotin Repressor: Modulation of Allostery by Corepressor Analogs.” Brown, P.H., Cronan, J.E., Grøtli, M. & Beckett, D. Journal of Molecular Biology, 337(4), pp. 857–869, 2004, Apr. doi: 10.1016/j.jmb.2004.01.041.
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“Effects of Protein Kinase C Dependent Phosphorylation and a Familial Hypertrophic Cardiomyopathy-Related Mutation of Cardiac Troponin I on Structural Transition of Troponin C and Myofilament Activation.” Kobayashi, T., Dong, W.-J., Burkart, E.M., Cheung, H.C. & Solaro, R.J. Biochemistry, 43(20), pp. 5996–6004, 2004, Apr. doi: 10.1021/bi036073n.
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“Cdc42 Regulates the Par-6 PDZ Domain through an Allosteric CRIB-PDZ Transition.” Peterson, F.C., Penkert, R.R., Volkman, B.F. & Prehoda, K.E. Molecular Cell, 13(5), pp. 665–676, 2004, Mar. doi: 10.1016/s1097-2765(04)00086-3.
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“Conserved Cysteine 126 in Triosephosphate Isomerase Is Required Not for Enzymatic Activity but for Proper Folding and Stability.” González-Mondragón, E., Zubillaga, R.A., Saavedra, E., Chánez-Cárdenas, M.E., Pérez-Montfort, R. & Hernández-Arana, A. Biochemistry, 43(11), pp. 3255–3263, 2004, Feb. doi: 10.1021/bi036077s.
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“Liver Fatty Acid-Binding Protein Colocalizes with Peroxisome Proliferator Activated Receptor α and Enhances Ligand Distribution to Nuclei of Living Cells.” Huang, H., Starodub, O., Mcintosh, A., Atshaves, B.P., Woldegiorgis, G., Kier, A.B. & Schroeder, F. Biochemistry, 43(9), pp. 2484–2500, 2004, Feb. doi: 10.1021/bi0352318.
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“Spectroscopic Studies of the Intermolecular Interactions of Congo Red and Tinopal CBS with Modified Cellulose Fibers.” Yamaki, S.B., Barros, D.S., Garcia, C.M., Socoloski, P., Oliveira,, O.N. & Atvars, T.D.Z. Langmuir, 21(12), pp. 5414–5420, 2005, May. doi: 10.1021/la046842j.
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“Photophysical study of a conjugated–non-conjugated PPV-type electroluminescent copolymer.” Machado, A., Neto, J.D.M., Cossiello, R., Atvars, T., Ding, L., Karasz, F. & Akcelrud, L. Polymer, 46(8), pp. 2452–2460, 2005, Mar. doi: 10.1016/j.polymer.2005.02.007.
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“White polymer light-emitting devices from ternary-polymer blend with concentration gradient.” Park, J.H., Lee, T.-W., Kim, Y.C., Park, O.O. & Kim, J.K. Chemical Physics Letters, 403(4-6), pp. 293–297, 2005, Feb. doi: 10.1016/j.cplett.2004.12.119.
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“Hole-transporting property of a chemically hybridized poly(vinylcarbazole)-fullerene.” Park, J.H., Park, O.O., Kim, J., Yu, J.-W., Kim, J.K. & Kim, Y.C. Current Applied Physics, 4(6), pp. 659–662, 2004, Nov. doi: 10.1016/j.cap.2003.11.066.
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“Photophysical Study in Blends of Poly(alkyl methacrylate-co-styrene)/Polystyrene.” de Andrade, M.L. & Atvars, T.D.Z. Macromolecules, 37(24), pp. 9096–9108, 2004, Nov. doi: 10.1021/ma049265e.
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“Diffusion of a Single Component in a Binary Polymer Blend upon Annealing Monitored by Fluorescence Microspectroscopy.” de Andrade, M.L. & Atvars, T.D.Z. Macromolecules, 37(25), pp. 9626–9630, 2004, Nov. doi: 10.1021/ma048873r.
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“Optoelectronic and Photophysical Properties of Polyfluorene Blends as Side-Chain Length and Shape.” Byun, H.Y., Chung, I.J., Shim, H.-K. & Kim, C.Y. Macromolecules, 37(18), pp. 6945–6953, 2004, Aug. doi: 10.1021/ma049772w.
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“The effects of alkyl side-chain length and shape of polyfluorenes on the photoluminescence spectra and the fluorescence lifetimes of polyfluorene blends with poly(n-vinylcarbazole).” Byun, H.Y., Chung, I.J., Shim, H.-K. & Kim, C.Y. Chemical Physics Letters, 393(1-3), pp. 197–203, 2004, Jul. doi: 10.1016/j.cplett.2004.06.029.
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“Enhanced light output in bilayer light-emitting diodes with film thickness variations.” Park, J.H., Lee, K.-J., Park, O.O., Yu, J.-W., Kim, Y.C. & Kim, J.K. Chemical Physics Letters, 386(1-3), pp. 101–104, 2004, Mar. doi: 10.1016/j.cplett.2004.01.039.
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“Dynamic and Static Fluorescence Spectroscopy Applied to Miscibility of Poly(n-butyl methacrylate-co-styrene) with Polystyrene and Morphological Analysis by Epifluorescence Microscopy.” Andrade, M.L.d. & Atvars, T.D.Z. The Journal of Physical Chemistry B, 108(13), pp. 3975–3984, 2004, Mar. doi: 10.1021/jp034664h.
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“Surface Treatment to Enhance the Quantum Efficiency of Semiconductor Nanocrystals.” Jang, E., Jun, S., Chung, Y. & Pu, L. The Journal of Physical Chemistry B, 108(15), pp. 4597–4600, 2004, Mar. doi: 10.1021/jp049475t.
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“Photo and electroluminescence studies of poly(methyl methacrylate-co-9-anthryl methyl methacrylate).” Deus, J.F.d., Andrade, M.L., Atvars, T.D. & Akcelrud, L. Chemical Physics, 297(1-3), pp. 177–186, 2004, Feb. doi: 10.1016/j.chemphys.2003.10.023.
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“Orbital Control of the Color and Excited State Properties of Formylated and Fluorinated Derivatives of Azulene.” Shevyakov, S.V., Li, H., Muthyala, R., Asato, A.E., Croney, J.C., Jameson, D.M. & Liu, R.S.H. The Journal of Physical Chemistry A, 107(18), pp. 3295–3299, 2003, Apr. doi: 10.1021/jp021605f.
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“Immobilized Particle Arrays: Coalescence of Planar- and Suspension-Array Technologies.” Stevens, P.W., Wang, C.H.J. & Kelso, D.M. Analytical Chemistry, 75(5), pp. 1141–1146, 2003, Jan. doi: 10.1021/ac020580d.
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