ChronosDFDFast lifetime measurements

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Overview of ChronosDFD

ChronosDFD for lifetime measurements in complex decays in less than 1 second is fully-automated through Vinci, a user-friendly, Windows-based software package.

Key Features of ChronosDFD

Frequency Domain Measurements

Frequency Domain Measurements

Maximum Sensitivity

Maximum Sensitivity

Fully Automated

Fully Automated

Integration of External Devices

Integration of External Devices

Upgradeable

Upgradeable

  • Flexible instrument configuration with a variety of light sources (laser diodes, LEDs and Ti:Sapphire laser)
  • A compact footprint and short optical path length for maximum sensitivity and efficient light coupling into the sample
  • Second to picosecond lifetime measurement capabilities
  • Full automation of instrument components including: cuvette holder, polarizers, shutters, filterwheel, monochromators and stirrers
  • PC-controlled integration of temperature path, titrator, stopped-flow apparatus and pressure pump
  • Upgradable to a full steady-state instrument
  • T-format and parallel beam optical design for fast and precise polarization measurements
  • Powered by Vinci-Multidimensional Fluorescence Spectroscopy

Key Characteristics of Data Acquisition via Frequency-domain with ChronosDFD

  • Fluorescence lifetime is calculated from two measureable parameters: phase angle and modulation
  • Is a faster and less artifact-prone
  • Allows one step measurements of anisotropy decays (rotational correlation times)
  • Is better at resolving short lifetime contributions
  • Is the method of choice for lifetime-based sensing and real-time measurements because of high sampling rates in the millisecond time scale

The Data is Clear!

Anisotropy Decays Graph of Fluorescein in Propylene Glycol

Fluorescein in Propylene Glycol

Frequency-domain anisotropy decays (differential polarized phase angle and amplitude ratio) of fluorescein in propylene glycol measured on ChronosDFD using an excitation wavelength of 470 nm (Xenon arc lamp). The emission was collected using a 530 longpass filter. Calculated values for θ = 5.3 ns with R0 = 0.40 and τ = 4 ns, T = 27-28°C.
Phase and Modulation Graph of BodipyFL in Water

BodipyFL in Water

Frequency responses (phase and modulation) of BodipyFL in water acquired on ChronosDFD using a 471 nm laser diode. The emission was collected through a 520 longpass filter. The data is best fitted with a single exponential decay time of 5.87 ns (χ2 = 0.97).

Product Specifications for ChronosDFD

Light Source
  • Laser diodes (nm): 370, 405, 436, 473, 635, 690, 780, 830
  • LEDs (nm): 280, 300, 335, 345, 460, 500, 520
  • Pulsed Lasers: Supercontinuum, Ti:Sapphire, Pulsed Laser Diodes
Focusing & Collection Geometry
  • Parallel beam design for precise polarization measurements
Polarizers
  • UV grade Glan-Thompson with L/A=2.0
Detectors
  • Fast PMT
  • hybrid PMT
  • APD
Detection Modes
  • Photon counting electronics
Wavelength Range
  • 200 nm to 1700 nm (detector dependent)
Max Counts Range
  • Up to 80 million counts/s (using hybrid detectors)
Lifetime Measurements Range
  • 10-12 s to 1 s
Operating System
  • Windows 10
Power Requirements
  • Universal power input: 110 - 240 V, 50/60 Hz, 400 VAC
Dimensions (mm)
  • 540 (L) x 425 (W) x 235 (H)
Weight
  • 25 kg
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Configuration for ChronosDFD

Configuration of ChronosDFD

Product Accessories for ChronosDFD

Product Software for ChronosDFD

Vinci

Vinci

A comprehensive multidimensional fluorescence spectroscopy software program designed to enhance the capabilities and performance of ISS spectrofluorometers.

Learn More

Product Resources